Effect of high glucose concentration on the synthesis of monocyte chemoattractant protein-1 in human peritoneal mesothelial cells: Involvement of protein kinase C

Human peritoneal mesothelial cells (HMC) contribute to the activation and control of inflammatory processes in the peritoneum by their potential to produce various inflammatory mediators. The present study was designed to assess the effect of glucose, the osmotic active compound in most commercially available peritoneal dialysis fluids, on the synthesis of the C-C chemokine monocyte chemoattractant protein-1 (MCP-1) in cultured HMC. The MCP-1 concentration in the cell supernatants was determined by enzyme-linked immunosorbent assay and the MCP-1 mRNA expression was examined using Northern blot analysis. Incubation of HMC with glucose (30-120 mM) resulted in a time- and concentration-dependent increase in MCP-1 protein secretion and mRNA expression. After 24 h the MCP-1 synthesis was increased from 2.8 +/- 0.46 to 4.2 +/- 0.32 ng/10(5) cells (n = 5, p 2001 S. Karger AG. Basel

Thermodynamics of a model for RNA folding

We analyze the thermodynamic properties of a simplified model for folded RNA molecules recently studied by G. Vernizzi, H. Orland, A. Zee (in {\it Phys. Rev. Lett.} {\bf 94} (2005) 168103). The model consists of a chain of one-flavor base molecules with a flexible backbone and all possible pairing interactions equally allowed. The spatial pseudoknot structure of the model can be efficiently studied by introducing a $N \times N$ hermitian random matrix model at each chain site, and associating Feynman diagrams of these models to spatial configurations of the molecules. We obtain an exact expression for the topological expansion of the partition function of the system. We calculate exact and asymptotic expressions for the free energy, specific heat, entanglement and chemical potential and study their behavior as a function of temperature. Our results are consistent with the interpretation of $1/N$ as being a measure of the concentration of $\rm{Mg}^{++}$ in solution.Comment: 11 pages, 4 figure

GeoCLEF 2007: the CLEF 2007 cross-language geographic information retrieval track overview

GeoCLEF ran as a regular track for the second time within the Cross Language Evaluation Forum (CLEF) 2007. The purpose of GeoCLEF is to test and evaluate cross-language geographic information retrieval (GIR): retrieval for topics with a geographic specification. GeoCLEF 2007 consisted of two sub tasks. A search task ran for the third time and a query classification task was organized for the first. For the GeoCLEF 2007 search task, twenty-five search topics were defined by the organizing groups for searching English, German, Portuguese and Spanish document collections. All topics were translated into English, Indonesian, Portuguese, Spanish and German. Several topics in 2007 were geographically challenging. Thirteen groups submitted 108 runs. The groups used a variety of approaches. For the classification task, a query log from a search engine was provided and the groups needed to identify the queries with a geographic scope and the geographic components within the local queries

Surveillance of an Online Social Network to Assess Population-level Diabetes Health Status and Healthcare Quality

Objective: Test a novel health monitoring approach by engaging an international online diabetes social network (SN) in consented health surveillance. Methods: Collection of structured self-reports about preventive and self-care practices and health status using a software application (“app”) that supports SN-mediated health research. Comparison of SN measures by diabetes type; and, SN with Behavioral Risk Factor Surveillance System (BRFSS) data, for US-residing insulin dependent respondents, using logistic regression. Results: Of 2,414 SN app users, 82% (n=1979) provided an A1c and 41% (n=996) completed a care survey of which 931 have diabetes. Of these: 65% and 41% were immunized against influenza and pneumonia respectively, 90% had their cholesterol checked, 82% and 66%, had their eyes and feet checked, respectively. Type 1/LADA respondents were more likely than Type 2/pre-diabetic respondents to report all five recommended practices (Adjusted OR (95% CI) 2.2 (1.5, 3.2)). Past year self-care measures were: 58% self-monitored their blood glucose (SMBG) ≥ 5 times daily, 37% saw their diabetes nutritionist, 56% saw a diabetes nurse educator, 53% saw a doctor for their diabetes ≥ 4 times. Reports of health status did not differ by diabetes type in the SN sample. The SN group was more likely than the BRFSS comparator group to use all five preventive care practices (Adjusted OR (95% CI) 1.8 (1.4, 2.1) and SMBG ≥ 5 times daily (Adjusted OR (95% CI) 10.1 (6.8, 14.9). Conclusions: Rapid assessment of diabetes care practices using a novel, SN-mediated approach can extend the capability of standard health surveillance systems

GeoCLEF 2006: the CLEF 2006 Ccross-language geographic information retrieval track overview

After being a pilot track in 2005, GeoCLEF advanced to be a regular track within CLEF 2006. The purpose of GeoCLEF is to test and evaluate cross-language geographic information retrieval (GIR): retrieval for topics with a geographic specification. For GeoCLEF 2006, twenty-five search topics were defined by the organizing groups for searching English, German, Portuguese and Spanish document collections. Topics were translated into English, German, Portuguese, Spanish and Japanese. Several topics in 2006 were significantly more geographically challenging than in 2005. Seventeen groups submitted 149 runs (up from eleven groups and 117 runs in GeoCLEF 2005). The groups used a variety of approaches, including geographic bounding boxes, named entity extraction and external knowledge bases (geographic thesauri and ontologies and gazetteers)

Challenges to evaluation of multilingual geographic information retrieval in GeoCLEF

This is the third year of the evaluation of geographic information retrieval (GeoCLEF) within the Cross-Language Evaluation Forum (CLEF). GeoCLEF 2006 presented topics and documents in four languages (English, German, Portuguese and Spanish). After two years of evaluation we are beginning to understand the challenges to both Geographic Information Retrieval from text and of evaluation of the results of geographic information retrieval. This poster enumerates some of these challenges to evaluation and comments on the limitations encountered in the first two evaluations

Willingness to share personal health record data for care improvement and public health: A survey of experienced personal health record users

Background: Data stored in personally controlled health records (PCHRs) may hold value for clinicians and public health entities, if patients and their families will share them. We sought to characterize consumer willingness and unwillingness (reticence) to share PCHR data across health topics, and with different stakeholders, to advance understanding of this issue. Methods: Cross-sectional 2009 Web survey of repeat PCHR users who were patients over 18 years old or parents of patients, to assess willingness to share their PCHR data with an-out-of-hospital provider to support care, and the state/local public health authority to support monitoring; the odds of reticence to share PCHR information about ten exemplary health topics were estimated using a repeated measures approach. Results: Of 261 respondents (56% response rate), more reported they would share all information with the state/local public health authority (63.3%) than with an out-of-hospital provider (54.1%) (OR 1.5, 95% CI 1.1, 1.9; p = .005); few would not share any information with these parties (respectively, 7.9% and 5.2%). For public health sharing, reticence was higher for most topics compared to contagious illness (ORs 4.9 to 1.4, all p-values < .05), and reflected concern about anonymity (47.2%), government insensitivity (41.5%), discrimination (24%). For provider sharing, reticence was higher for all topics compared to contagious illness (ORs 6.3 to 1.5, all p-values < .05), and reflected concern for relevance (52%), disclosure to insurance (47.6%) and/or family (20.5%). Conclusions: Pediatric patients and their families are often willing to share electronic health information to support health improvement, but remain cautious. Robust trust models for PCHR sharing are needed

Two-electron atoms, ions and molecules

The quantum mechanics of two-electron systems is reviewed, starting with the ground state of the helium atom and helium-like ions, with central charge $Z\ge 2$. For Z=1, demonstrating the stability of the negative hydrogen ion, H$^-$, cannot be achieved using a mere product of individual electron wave functions, and requires instead an explicit account for the anticorrelation among the two electrons. The wave function proposed by Chandrasekhar is revisited, where the permutation symmetry is first broken and then restored by a counter-term. More delicate problems can be studied using the same strategy: the stability of hydrogen-like ions $(M^+,m^-,m^-)$ for any value of the proton-to-electron mass ratio $M/m$; the energy of the lowest spin-triplet state of helium and helium-like ions; the stability of the doubly-excited hydrogen ion with unnatural parity. The positronium molecule $(e^+,e^+,e^-,e^-)$, which has been predicted years ago and discovered recently, can also be shown to be stable against spontaneous dissociation, though the calculation is a little more involved. Emphasis is put on symmetry breaking which can either spoil or improve the stability of systems.Comment: 16 pages, 2 figure

Genetic variability and incidence of systemic diseases in wild vines (Vitis vinifera ssp. silvestris) along the Danube

In the riparian woods of Danube and March east of Vienna 87 wild specimens of Vitis vinifera ssp. silvestris were genetically analysed and compared. The silvestris population can be split into 6 distinct groups, but this clustering cannot be explained solely by the geographical distance. The unique genetic variability observed represents a strong case for preservation of wild grapevines.The incidence of bacterioses, viroses and nematodes transmitting nepoviruses to these vines were registered. None of the analysed specimens suffered from Agrobacterium vitis-induced crown gall. Only some vines were infected by viral pathogens such as GLRaV I and SLRV. Thus the wild vines do not constitute a risk for the surrounding commercial vineyards. On the other hand, diseases spread from cultivated grapevines may seriously harm the wild vine population. Four species of nematodes transmitting nepoviruses were registered. Samples of Xiphinema vuittenezi and Longidorus attenuatus from the Lobau (natural forests, north of the Danube in the area of Vienna) differ morphometrically from others found on arable soils or isolated from the research area.